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KMID : 0363320190400030356
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Journal of Korean Oriental Internal Medicine
2019 Volume.40 No. 3 p.356 ~ p.368
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Effects of Pharbitidis Semen on 3T3-L1 Cells and Obesity-induced Rats
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Shin Man-Ho
Lee Da-Eun
Jang Hye-Yeon
Lee Young-Su
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Abstract
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Objectives: This study was designed to evaluate the efficacy of Pharbitidis Semen (PS) on obesity using 3T3-L1 cells and high-fat-diet rats.
Methods: After the administration of PS at a concentration of 0.125, 0.25, and 0.50 mg/ml in 3T3-L1 cells, the degree of differentiation of adipocytes was measured by Oil Red O staining. The experimental animals were divided into five groups in vivo. Normal group: diet-fed normal group (Normal), Control group (Control): high-fat-diet (HFD)-fed, Positive control group (PC): HFD+Contrave 200 mg/kg, PS_Low group: HFD+PS 250 mg/kg group, PS_High group: HFD+PS 500 mg/kg.
Results: The total cholesterol, low density lipoprotein-cholesterol (LDL-cholesterol), and triglycerides (TG) content of the PS medication groups were significantly lower than those of the control group. In addition, high density lipoprotein cholesterol (HDL-cholesterol) tended to increase, and free fatty acid, total lipid, and phospholipid tended to decrease, but the decrease was not statistically significant. The administration of PS inhibited the differentiation of 3T3-L1 adipocytes, and a statistically significant difference was found in the group administered with 0.125 mg/ml. The mRNA expression of PPAR-γ and leptin, which are involved in the differentiation of adipocytes, was observed using reverse transcription polymerase chain reaction (RT-PCR). The expressions of PPAR-γ and leptin significantly decreased in comparison with the control and the positive control.
Conclusions: PS can be applied clinically against obesity.
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KEYWORD
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Pharbitidis Semen, 3T3-L1 cell, leptin, adiponectin, PPAR-γ
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